Discussion
The sequencing of ..................... helped investigators conclude that all living things can be classified into three domains.
Answer: C
The sequencing of rRNA led to the three domains.
Carl Woese and George Fox compared sequences of small-subunit ribosomal RNA (16S in prokaryotes) in the 1970s. rRNA suits this because every cellular organism has it, it performs the same function everywhere, and it changes slowly. The comparisons showed that methanogens and their relatives were as distinct from bacteria as from eukaryotes, giving the domains Bacteria, Archaea and Eukarya.
DNA in general was not the marker used. Cytochrome c and haemoglobin are proteins limited to some lineages (haemoglobin to a few) and were used for trees within eukaryotes, not to define domains.
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