Discussion
Which enzyme of the following is used as a marker for lysosomal fraction ?
Answer: C
The lysosomal marker enzyme is acid phosphatase.
In cell fractionation, each organelle is traced by an enzyme found only in it. Lysosomes contain acid hydrolases that work at their internal pH of about 5, and acid phosphatase is the classic marker. It was the enzyme whose latent activity led Christian de Duve to discover lysosomes.
Succinate dehydrogenase, of the inner mitochondrial membrane, is the marker for mitochondria, and pyruvate dehydrogenase is also mitochondrial. Phospholipases occur in many compartments and are not used as a lysosomal marker.
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