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In bio-gas production, which is an anaerobic digestion, the correct sequence of stages in the process is-

  • A.Methanogenesis → Hydrolysis → Acidification
  • B.Acidification → Methanogenesis → Hydrolysis
  • C.Hydrolysis → Acidification → Methanogenesis
  • D.Hydrolysis → Methanogenesis → Acidification

Answer: C

The correct sequence is Hydrolysis → Acidification → Methanogenesis.

In anaerobic digestion, bacteria first hydrolyse complex organic matter (carbohydrates, proteins and fats) into simple soluble compounds. Acid-forming bacteria then ferment these into volatile fatty acids, acetic acid, hydrogen and carbon dioxide (acidification, also split into acidogenesis and acetogenesis). Finally methanogenic archaea convert acetate, hydrogen and carbon dioxide into methane.

Methanogens can only use the simple products of the earlier stages, so any order that puts methanogenesis before hydrolysis or acidification is wrong.

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