Discussion
Which of the following test is used for fat estimation ?
Answer: D
Fat is detected with the ethanol emulsion test.
The sample is shaken with ethanol, in which fats dissolve, and the liquid is then poured into water. If fat is present it comes out of solution as fine droplets, giving a cloudy white emulsion.
The other tests detect other nutrients. The iodine test turns blue-black with starch. Benedict's test gives a brick-red precipitate with reducing sugars. The biuret test turns violet with proteins.
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