Discussion
The most sensitive optical detector coupled with high performance liquid chromatographic system is :
Answer: C
The most sensitive optical detector for HPLC is the fluorescence detector.
A fluorescence detector measures the light emitted by excited molecules against a dark background, so it is often 10 to 1,000 times more sensitive than UV absorbance detection, and very selective, for compounds that fluoresce naturally or after derivatisation.
UV and photodiode array detectors measure absorbance, which is less sensitive. A mass detector can be very sensitive, but it is not an optical detector, so it does not answer the question.
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