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The sequence :

Mobile phase delivery system → pump → injector → column → detector → data processing system, is associated with which of the instrumental set up.

  • A.GC
  • B.HPLC
  • C.HPTLC
  • D.CZE

Answer: B

The sequence describes HPLC, high performance liquid chromatography.

In HPLC the liquid mobile phase is drawn from its reservoir and delivery system and forced by a high-pressure pump through the injector, where the sample enters, into a packed column, where the separation happens. The separated components pass through a detector (often UV), and its signal goes to a data system that produces the chromatogram.

Gas chromatography uses a carrier gas from a cylinder, not a pump. HPTLC separates on a plate with no pump or column. Capillary zone electrophoresis drives separation by an electric field in a capillary, not by a pump.

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