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In planar chromatography Rf is calculated by :

  • A.Distance travelled by solvent front / Distance travelled by solute
  • B.Distance to centre of component spot / Distance to centre of standard spot
  • C.Distance to centre of standard spot / Distance to centre of component spot
  • D.Distance travelled by solute / Distance travelled by solvent front

Answer: D

Rf is the distance travelled by the solute divided by the distance travelled by the solvent front.

Both distances are measured from the origin line where the sample was spotted:

\[ R_{f} = \frac{d_{\text{solute}}}{d_{\text{solvent front}}} \]

Because the solute can never move further than the solvent, Rf always lies between 0 and 1. Option A is the reciprocal, which would always be 1 or more. The ratios using a standard spot define a relative value (Rx or Rst), used to compare a component with a reference, not Rf.

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