Discussion
Separation of two compounds A and B is done by reverse phase chromatography. If A is more polar than B, which statement is correct ?
Answer: A
In reverse phase chromatography A will elute followed by B.
Reverse phase uses a non-polar stationary phase (typically C18 bonded silica) and a polar mobile phase such as water with methanol or acetonitrile. A polar compound is attracted to the polar mobile phase and spends little time on the non-polar packing, so it comes out first. The less polar compound B is held longer by the stationary phase and elutes after A.
B first would be the order in normal phase chromatography, where the stationary phase is polar. Two compounds of different polarity do not co-elute, and one compound does not prevent the elution of another.
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