Discussion
The process of separation based on the differential rate of migration of charged species in a buffer solution across which an electric field is applied is known as :
Answer: D
The technique is electrophoresis.
In electrophoresis an electric field is applied across a buffer, and charged species move towards the electrode of opposite charge at rates that depend on their charge, size and shape. Differences in that rate of migration separate them, which is how DNA fragments, serum proteins and isoenzymes are separated in forensic work.
The chromatographic options separate by partition or exchange between a stationary and a mobile phase. Ion chromatography uses an ion-exchange resin, paper chromatography a liquid held on paper, and micellar liquid chromatography a surfactant mobile phase; none of them is driven by an applied field.
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