Discussion
Match the following term used in chromatography with their abbreviation :
| List I | List II |
|---|---|
| (a) N | (i) Dead time |
| (b) B/A | (ii) Capacity factor |
| (c) K | (iii) Retention time |
| (d) RT | (iv) Asymmetry factor |
| (v) Efficiency |
Answer: B
The correct match is a-v, b-iv, c-ii, d-iii.
- N, the number of theoretical plates, measures column efficiency.
- B/A, the ratio of the back and front half-widths of a peak at 10% of its height, is the asymmetry factor.
- K (k') is the capacity factor or retention factor, how long a compound is retained relative to the dead time.
- RT is the retention time.
Dead time (tM or t0) is the time an unretained compound takes and is the extra item in List II. Every other option gives N or K the dead time or swaps B/A and N, which is why they fail.
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