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Method for fragmenting molecules in tandem mass spectroscopy is known as :

  • A.Mass Induced Dissociation
  • B.Collision Induced Dissociation
  • C.Charge Induced Dissociation
  • D.Impact Induced Dissociation

Answer: B

The method is collision induced dissociation (CID).

In tandem mass spectrometry (MS/MS) the first analyser selects a precursor ion. That ion is accelerated into a collision cell containing an inert gas such as argon, nitrogen or helium, where collisions convert part of its kinetic energy into internal energy and break it into product ions, which the second analyser then separates. The product ion pattern identifies the molecule with high specificity, which is why MS/MS is used to confirm drugs and toxins.

The other names are not the standard technique. Fragmentation is caused by collision with gas atoms, not by mass, charge or a generic 'impact'.

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