Discussion
In HPLC, LOD and LOQ are obtained using :
Answer: C
LOD and LOQ are obtained from the signal to noise ratio.
The limit of detection is the smallest amount of analyte that gives a peak clearly distinguishable from baseline noise, conventionally a signal-to-noise ratio of about 3:1. The limit of quantitation is the smallest amount that can be measured with acceptable precision and accuracy, conventionally about 10:1.
Retention time, column efficiency, peak asymmetry and dead time describe chromatographic separation and peak shape, not the sensitivity limits of a method.
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