Discussion
Analysis of a drug by UHPLC generates a pressure of 14000 psi. A column with which of the following particle size is most suitable for analysis :
Answer: B
A column with 2 µm particles is the most suitable.
Ultra-high-performance liquid chromatography uses columns packed with very small particles, typically below 2 micrometres, which give much higher efficiency and faster separations. Back-pressure rises steeply as particle size falls, so these columns need pumps that work at around 15,000 psi or more; a pressure of 14,000 psi is exactly what a sub-2 micrometre column generates.
Particles of 5 and 10 micrometres are the sizes of conventional HPLC, run at a few thousand psi, and 20 micrometres is used for preparative work. None of them needs UHPLC pressure.
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