Discussion
The examination of diatoms from bone marrow/water is performed most commonly by :
Answer: D
The key's answer is Both A and C: acid digestion and the Soluene-350 method.
In suspected drowning, diatoms are looked for in bone marrow (usually from the femur) and compared with diatoms in the water. Because their walls are silica, they survive harsh treatment. The classic method is acid digestion, boiling the tissue in concentrated nitric acid (sometimes with sulphuric acid) to destroy organic matter and leave the diatom frustules. Soluene-350, a tissue solubiliser, dissolves the tissue more gently and is the other widely used method.
A sodium sulphate method is not one of the standard diatom extraction procedures, so the option naming the two common methods together is the one the key accepts.
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