Discussion
Which of the following is correct for the disadvantages of RFLP technqiue ?
I. Takes a lot of time
II. Requires large intact sample
III. Produces large (30,000 BP) DNA strand.
Answer: D
All three are disadvantages, so I, II and III are correct.
RFLP analysis (restriction fragment length polymorphism) involves digesting DNA, separating the fragments on a gel, Southern blotting and probing, which takes days or weeks (I). It needs a relatively large amount of intact, high-molecular-weight DNA, so degraded samples often fail (II). And it works with long fragments, thousands to tens of thousands of base pairs, which are harder to resolve precisely than the short products used in modern STR typing (III).
Since every statement is a drawback, the options with only two of them are incomplete.
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