Discussion
Venous blood collected as a reference blood sample should be collected in :
Answer: B
Reference blood should go in purple-topped vacutainer EDTA tubes.
EDTA (ethylenediaminetetraacetic acid) is an anticoagulant that chelates calcium, which also inhibits the DNases that degrade DNA, so whole blood collected in EDTA is the standard reference sample for DNA profiling. Tubes containing EDTA are identified by a purple (lavender) stopper.
Green tops contain heparin, which inhibits PCR, and yellow tops contain ACD or are used for blood cultures. Pink tops also hold EDTA but are used mainly in blood banking for cross-matching, so the standard forensic reference tube is the purple one.
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