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Among the following which is more suitable to determine the species origin of blood stains ?

  • A.Precipitin tube technique
  • B.Crose-over electrophoresis
  • C.Latex agglutinins
  • D.Anti-globulin consumption test

Answer: B

The more suitable technique is cross-over electrophoresis.

Species origin is determined by the reaction between proteins in the stain and an antiserum against a particular species. In cross-over (counter) immunoelectrophoresis, an electric field drives the antigen and antibody towards each other through a gel, so they meet and form a precipitin line quickly and with much greater sensitivity than when they diffuse on their own. It works on old and small stains and uses little antiserum.

The precipitin tube (ring) test relies on the same reaction but is slower and less sensitive. Latex agglutination and the antiglobulin consumption test are used for other serological purposes, not as the method of choice for species identification.

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