Discussion
Brentamine Fast Blue-B is used to test :
Answer: A
Brentamine Fast Blue B is used to test seminal stains.
It is the colour reagent of the acid phosphatase test. Seminal acid phosphatase cleaves a substrate such as alpha-naphthyl phosphate to release naphthol, which couples with Brentamine Fast Blue B to give an intense purple colour within seconds. The speed and intensity of the reaction indicate semen, which contains far more acid phosphatase than any other body fluid.
Vaginal fluid has only low acid phosphatase activity and gives a slow, weak reaction. Saliva is identified by its amylase (starch-iodine or Phadebas tests) and urine by urea or creatinine tests.
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