Discussion
The modified version of the organic extraction method that separates epithelial and sperm cells from vaginal swab is :
Answer: A
The method is differential extraction.
Sperm heads are protected by disulphide-rich protein, so they resist lysis. In differential extraction the swab is first digested with proteinase K and detergent without a reducing agent: the vaginal epithelial cells burst, and their DNA goes into the female fraction. The intact sperm are then spun down and lysed with dithiothreitol (DTT) added, giving a male fraction. This separates the victim's and the assailant's DNA.
Microwave-assisted, reflux and Soxhlet extraction are chemical extraction methods for organic compounds, not ways of separating cell types.
Post your comment
Please log in or register to post a comment.
Examination Categories
- SSC Exams
- Banking Exams
- Teaching Exams
- Civil Services Exams
- Railways Exams
- Engineering Recruitment Exams
- Defence Exams
- State Government Exams
- Police Exams
- Insurance Exams
- Nursing Exams
- Other Government Exams
- NRA CET
- PG Entrance Exams
- Campus Placement
- MBA Entrance Exams
- CUET and UG Entrance
- Judiciary Exams
- Regulatory Body Exams
- Software Development