Discussion
The most suitable solvent system for chromatography of blood sample is
Answer: C
The solvent system given as most suitable for chromatography of a blood sample is methanol, acetic acid and water in the ratio 90 : 3 : 7. Blood contains polar substances such as proteins and pigments, which need a polar solvent mixture with a little acid to run as compact spots. The other mixtures listed are either much less polar, as in chloroform with ethanol, or are different proportions of polar solvents, such as 70 : 30 methanol and water with no acid or ethanol with acetic acid and water at 92 : 1 : 7. Of the four, only option C has both the strongly polar methanol and water base and a small, acidic share of acetic acid, so it is the keyed answer.
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