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Thermal cycler is an instrument that is used in the :

  • A.Detection of blood
  • B.Amplification of DNA
  • C.Detection of semen stains
  • D.Separation of plasma from blood

Answer: B

A thermal cycler is used in the amplification of DNA.

The polymerase chain reaction copies a target DNA sequence through repeated cycles of denaturation (about 94-95°C), annealing of primers (about 50-65°C) and extension (about 72°C). A thermal cycler changes the temperature of the reaction tubes through these steps automatically, doubling the target with each cycle. Forensic STR profiling depends on this amplification.

Detection of blood or semen stains uses presumptive and confirmatory tests, and plasma is separated from blood by centrifugation, not by a thermal cycler.

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