Discussion
Spermine is detected in semen by which test ?
Answer: C
Spermine is detected by the Barberio test.
In Barberio's test a saturated solution of picric acid is added to a semen extract; spermine forms yellow needle-shaped crystals of spermine picrate, seen under the microscope. It is a classic presumptive test for semen.
The Florence test detects choline, giving brown crystals with iodine in potassium iodide. Fluorescence (under UV light) helps locate stains, ELISA is used for semen-specific proteins such as PSA, and agglutination inhibition is a blood or secretor grouping method. None of these is the spermine test.
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