Discussion
Isoamyl alcohol is added to phenol-chloroform mixture in organic extraction of DNA to :
Answer: C
Isoamyl alcohol is added to reduce foaming.
In organic extraction the lysed sample is shaken with phenol-chloroform-isoamyl alcohol, usually in the ratio 25:24:1. Isoamyl alcohol acts as an antifoaming agent during mixing, and it also helps the aqueous and organic phases separate cleanly, so the DNA in the upper aqueous phase can be drawn off.
It does not concentrate DNA; that is done later by ethanol precipitation. Extracting proteins out of the aqueous phase is the job of phenol, which denatures them and takes them into the organic phase. Quality improves as a result of the whole procedure, not because of the isoamyl alcohol.
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