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In seminal stains examination, DNA is present in :

  • A.Sperms
  • B.Seminal fluid
  • C.Tail
  • D.Prostate-specific antigen

Answer: A

In seminal stain examination the DNA comes from the sperm.

A spermatozoon carries its DNA in the nucleus of its head. Differential extraction exploits this: the victim's epithelial cells are lysed first, and the tough sperm heads are then broken with a reducing agent such as DTT to obtain the male DNA separately.

The seminal fluid itself is a largely cell-free secretion; in a man with no sperm, any DNA has to come from the few epithelial and white cells it carries. The sperm tail holds no nucleus, and prostate-specific antigen is a protein, not a source of DNA.

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