Discussion
Δ9-THC gives.................colour with Duquenois reagent.
Answer: A
Δ9-THC gives a violet colour with Duquenois reagent.
In the Duquenois-Levine test, the suspected cannabis is treated with Duquenois reagent (vanillin and acetaldehyde in ethanol) and then concentrated hydrochloric acid. Cannabinoids develop a blue-violet to purple colour. Chloroform is then added; if the violet colour moves into the chloroform layer, the result is taken as positive for cannabis.
Red, pink and orange are not the cannabinoid response. Other plant materials may give coloured products, but they do not pass the violet colour into the chloroform layer, which is the point of the Levine step.
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