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A peak elutes at \( 300 \ \mathrm{s} \) with a base width of \( 10 \ \mathrm{s} \). The plate number is

  • A.480
  • B.1600
  • C.14400
  • D.900

Answer: C

Applying the standard relation for a base-width measurement:

\[ N = 16\left(\frac{t_{R}}{w}\right)^{2} = 16(30)^{2} = 14400 \]

giving 14400. The factor of sixteen belongs to the base width; a half-height measurement uses 5.54 instead. Quoting which width was used is essential, since the two constants differ by nearly threefold and a plate number without its method is meaningless.

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