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What does column bleed refer to?

  • A.Air diffusing back into the column outlet
  • B.Stationary phase decomposing and entering the detector
  • C.Carrier gas leaking from a fitting
  • D.Sample carried over from a previous injection

Answer: B

At high temperature the stationary phase slowly degrades and its fragments reach the detector, raising the baseline as the oven is programmed upward. Leaks, carry-over and back-diffusion are separate faults. Because bleed rises steeply near a phase's maximum temperature, the rated limit is a real constraint rather than a cautious suggestion, and exceeding it shortens the column's life as well as spoiling the baseline. The quantity that varies and the quantity that does not are worth naming before any substitution is attempted.

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