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What does a slower temperature ramp achieve?

  • A.Greater sample capacity in the column
  • B.Better resolution at the cost of time
  • C.Faster analysis with the same resolution
  • D.Lower column bleed throughout the run

Answer: B

A gentler ramp gives each compound longer at a temperature where it partitions appreciably, so the peaks separate further at the price of a longer run. Speed falls rather than rises, bleed depends on the final temperature, and capacity is a column property. The ramp rate is the principal lever in method development once the phase is chosen, and it trades the two things the analyst usually cares about most. The quantity that varies and the quantity that does not are worth naming before any substitution is attempted.

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