Discussion
Why is a column conditioned before first use?
Answer: C
Heating under flow removes solvent and low molecular weight fragments left from the coating process, which would otherwise appear as a rising baseline for the first several runs. The coating is already applied, and flow and retention are set separately. A preparation step that trades an hour once against a spoiled baseline on every early run is worth the wait, and skipping it is a common cause of an apparently faulty new column. The quantity that varies and the quantity that does not are worth naming before any substitution is attempted.
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