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In prokaryotes, the sequence in mRNA that is recognized by the small subunit of ribosome during its translation is known as :

  • A.UAG sequence
  • B.Shine-Dalgarno sequence
  • C.Pribnow box sequence
  • D.AUG sequence

Answer: B

The sequence is the Shine-Dalgarno sequence.

In bacteria a purine-rich sequence (consensus AGGAGG) lies a few nucleotides upstream of the start codon on the mRNA. It pairs with a complementary sequence at the 3' end of the 16S rRNA in the small (30S) ribosomal subunit, which positions the ribosome so that the initiation codon sits in the P site.

AUG is the start codon itself, read by the initiator tRNA rather than recognised by base-pairing with rRNA. UAG is a stop codon. The Pribnow box (TATAAT) is a promoter element in DNA recognised by RNA polymerase, not a sequence in mRNA.

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