Discussion
The enzyme involved in the following reaction is...........
Amino acid + ATP + tRNA \(\rightleftharpoons\) aminoacyl tRNA + AMP + PPi
Answer: B
The enzyme is aminoacyl tRNA synthetase.
Aminoacyl tRNA synthetases charge tRNAs in two steps. The amino acid first reacts with ATP to form aminoacyl-AMP, releasing pyrophosphate; the activated amino acid is then transferred to the 2' or 3' hydroxyl of the tRNA's terminal adenosine, releasing AMP. The overall reaction is the one in the stem. Each of the twenty amino acids has its own synthetase, which is why they are called the second genetic code.
Peptidyl transferase forms peptide bonds on the ribosome, RNA polymerase makes RNA, and 'aminoacyl tRNA transferase' is not the name of this ligase.
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