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Which one of the following statements about batch culture of microbes is NOT correct?

  • A.Cells from stationary phase will show longer lag phase when inoculated in fresh growth medium compared to those collected from exponential phase.
  • B.Death phase of culture is often exponential in nature.
  • C.Stationary phase is the cryptic growth phase.
  • D.The rate of generation of new cells during exponential growth phase is constant.

Answer: D

The statement that is NOT correct is the rate of generation of new cells during exponential growth phase is constant.

In exponential growth the specific growth rate and the generation time are constant, but the population doubles every generation, so the number of new cells produced per unit time keeps increasing. A constant rate of producing new cells would describe linear, not exponential, growth.

The other statements are accepted. Stationary-phase cells need longer to adapt to fresh medium, so their lag phase is longer. Death often follows a logarithmic (exponential) decline. In stationary phase some cells grow on nutrients released by others that die, which is called cryptic growth.

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