Discussion
Which test is used to differentiate the members of Enterobacteriaceae?
Answer: D
The members of Enterobacteriaceae are differentiated by the IMViC test.
IMViC is a set of four biochemical tests: Indole, Methyl red, Voges-Proskauer and Citrate utilisation (the i is added for ease of saying). Their pattern separates the enteric bacteria, for example E. coli (++--) from Klebsiella and Enterobacter (--++).
The catalase test separates staphylococci from streptococci, and the coagulase test identifies Staphylococcus aureus. All Enterobacteriaceae are oxidase negative, so the oxidase test separates the family from organisms such as Pseudomonas but cannot tell its members apart.
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