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Why does an attenuated total reflectance objective improve spatial resolution?

  • A.The crystal's refractive index shortens the wavelength
  • B.The evanescent wave penetrates less deeply
  • C.The sample is compressed into a thinner layer
  • D.The detector is closer to the sample

Answer: A

Inside a high-index crystal the wavelength is shorter by the refractive index, so the diffraction limit is correspondingly finer - a germanium crystal of index four improves it fourfold. The shallow penetration limits depth rather than lateral resolution, and neither compression nor detector position is the mechanism. Working in a medium where the wavelength is shorter is the same trick immersion microscopy uses in the visible.

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