Discussion
What distinguishes reversed phase from normal phase chromatography?
Answer: A
In reversed phase the packing is non-polar and the eluent is polar, so polar compounds elute first and non-polar ones are retained - the opposite of the original normal phase arrangement on bare silica. The mobile phase is liquid in both, and nothing flows backwards. The name records only that the polarity relationship was inverted from what came first, which is worth knowing because the word suggests something far stranger than it is. Working one numerical case through both cases side by side fixes the distinction better than any rule of thumb.
No comment is present. Be the first to comment.
Loading…
Post your comment
Please log in or register to post a comment.
Examination Categories
- SSC Exams
- Banking Exams
- Teaching Exams
- Civil Services Exams
- Railways Exams
- Engineering Recruitment Exams
- Defence Exams
- State Government Exams
- Police Exams
- Insurance Exams
- Nursing Exams
- Other Government Exams
- NRA CET
- PG Entrance Exams
- Campus Placement
- MBA Entrance Exams
- CUET and UG Entrance
- Judiciary Exams
- Regulatory Body Exams
- Software Development