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What is isocratic elution?

  • A.A constant eluent composition throughout the run
  • B.A composition changing steadily from weak to strong during the run
  • C.A run at constant column temperature
  • D.A run at constant back pressure

Answer: A

Isocratic means the mobile phase composition is held fixed from injection to the last peak. A changing composition is gradient elution, and constant temperature and pressure are separate conditions that usually apply either way. An isocratic method is simpler, re-equilibrates instantly and transfers between instruments more reliably, which is why it is preferred wherever the sample's range of retention allows. Reading the definition literally, rather than recalling a formula, is the reliable route through questions of this shape.

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