Discussion
What is the drawback of a refractive index detector?
Answer: A
The signal is the difference in refractive index between sample and reference cells, and a changing eluent composition changes that difference enormously, so the baseline is unusable during a gradient. It is universal rather than selective, non-destructive, and needs no optical transparency. Being confined to isocratic work is a severe restriction, and it is why the detector survives mainly for sugars and polymers that nothing else sees. Naming the cost of a choice alongside its benefit is what turns a fact into a design decision.
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