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What limits the use of a fluorescence detector?

  • A.It cannot be used with gradient elution
  • B.It destroys the analyte during detection
  • C.It requires a completely transparent eluent
  • D.Few compounds fluoresce naturally

Answer: D

Natural fluorescence is uncommon, so the technique either suits a fortunate analyte or requires a derivatisation step to attach a fluorophore. Gradients are fine, nothing is destroyed, and eluent transparency is easily arranged. Extraordinary sensitivity available only for a minority of compounds is the trade, and derivatisation extends the range at the cost of another chemical step. Reading the definition literally, rather than recalling a formula, is the reliable route through questions of this shape.

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