Discussion
The back pressure rises steadily over several weeks. What is likely?
Answer: C
Particulates from samples and from pump seals accumulate on the frit at the column head, restricting the flow while the separation itself remains acceptable at first. Worn seals reduce pressure by leaking, phase loss reduces retention, and a dirty cell affects the baseline. That a gradual pressure rise points at a filter rather than at the column proper is worth knowing before an expensive column is discarded. Naming the cost of a choice alongside its benefit is what turns a fact into a design decision.
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