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The volume between where a gradient is formed and the column head is the ______ volume.

  • A.dead
  • B.extra-column
  • C.dwell
  • D.void

Answer: C

This is the dwell volume, and it delays the gradient's arrival at the column. Void and dead volume both describe the liquid held within the column itself, and extra-column volume is everything outside it that broadens peaks. Three volumes with three distinct effects, and confusing the dwell volume with the others is the commonest reason a gradient method fails to transfer between instruments. A limit that survives making every external component ideal is an intrinsic one, and worth knowing for that reason.

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