Discussion
Which of the following does not improve resolution?
Increasing the injection volume
Using a smaller particle size
Changing the organic modifier
Lengthening the column
Answer: D
A larger injection overloads the column and broadens or distorts the peaks, reducing resolution rather than improving it. The other three act on efficiency or selectivity. Because the resolution equation contains no term for sample amount, any change to it can only hurt - which is why injection volume is minimised subject to detecting the analyte.
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