Discussion
Why is a sample filtered before injection?
Answer: A
Anything larger than the packing's interstices lodges on the frit and raises the back pressure, eventually making the column unusable. Detection, concentration and degassing are unaffected by filtration. A two-minute step that protects a component costing several hundred pounds is among the best returns available in the laboratory, and skipping it is the commonest cause of a prematurely blocked column. Naming the cost of a choice alongside its benefit is what turns a fact into a design decision.
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