Discussion
Why might derivatisation be done before the column instead?
Answer: C
If the underivatised analyte is poorly retained or tails badly, converting it first can transform the chromatography as well as the detection. Reactors are readily available, reaction speed varies, and detectors tolerate reagents routinely. Choosing between pre- and post-column derivatisation means asking whether the problem lies in the separation or only in the detection, and the answer settles it immediately. A single measurement chosen to respond to the one asymmetry beats several that respond to shared behaviour.
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