Discussion
Why is a fast gradient not always faster overall?
Answer: B
A five-minute gradient followed by five minutes of re-equilibration is a ten-minute cycle, so the saving is half what the run time suggests. Detector settling and pump response are quick, and no cooling is required. Counting the cycle rather than the run is what makes a throughput comparison honest, and it is why a shallow gradient on a fast column sometimes beats a steep one on a slow column.
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