Discussion
What does an ion-pairing reagent do?
Answer: A
A long-chain ion of opposite charge pairs with the ionised analyte and lends it enough hydrophobic character to be retained on a C18 column. Buffering, chelation and absorbance suppression are other additives' functions. Making an analyte behave as though it had a property it lacks, rather than changing the column to suit it, is the technique - and the slow desorption is the price paid.
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