Discussion
Two proteins have the same molecular weight and same isoelectric point. The best way to resolve them would be by :
Answer: C
The best way is Reverse-phase chromatography.
Each method separates by one property. With identical molecular weight, gel filtration (size) cannot separate the two proteins. With identical isoelectric points, the methods that rely on charge, ion exchange chromatography and chromatofocusing (which separates by pI), also fail.
Reverse-phase chromatography separates by hydrophobicity, a property that depends on the amino acids exposed and is very unlikely to be the same in two different proteins, so it can resolve them.
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