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What does a mass spectrometer actually measure?

  • A.Mass-to-charge ratio
  • B.Molecular weight directly
  • C.The number of atoms in a molecule
  • D.The energy needed to ionise a molecule

Answer: A

Every analyser separates ions by the ratio of mass to charge, so a doubly charged ion of mass two hundred appears at a hundred. Molecular weight follows only once the charge is known, atom counts are inferred from the pattern, and ionisation energy is a separate quantity. That the axis is a ratio rather than a mass is what makes multiply charged ions both a complication and, in protein work, an opportunity. Most errors here come from carrying a result across from a neighbouring case where it does not hold.

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