Discussion
What is characteristic of an electrospray spectrum of a protein?
Answer: A
A protein acquires many protons and appears as a family of peaks differing by one charge, each at a much lower mass-to-charge ratio than the true mass. Deconvoluting that family gives the molecular weight with great precision. Little fragmentation occurs and the isotope pattern is a separate matter. That multiple charging brings a hundred-kilodalton protein within the range of an ordinary analyser is the technique's decisive advantage. The quantity that varies and the quantity that does not are worth naming before any substitution is attempted.
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