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Radioisotopes are frequently used in the study of cells. Assume a culture of E. coli is grown in a culture medium containing radioactive phosphorous. At the end of 48 hours, it is expected to find the radioactive label located in

  • A.enzymes
  • B.RNA
  • C.phospholipids
  • D.all of these

Answer: D

Correct answer: all of these

Phosphorus is a constituent of many cell components, so a 32P label turns up in all of them: in nucleic acids through the phosphate backbone of RNA and DNA, in phospholipids of the membranes, and in enzymes both as phosphorylated residues and through cofactors such as ATP, NAD and FAD.

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