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Which enzyme is responsible for biological nitrogen fixation ?

  • A.Nitrate reductase
  • B.Nitrogenase
  • C.Nitrite oxidase
  • D.Urease

Answer: C

Correct answer: Nitrogenase

Nitrogenase reduces atmospheric N2 to ammonia, an energy-expensive reaction requiring around 16 ATP per molecule fixed. The enzyme is irreversibly inactivated by oxygen, which is why nitrogen-fixing cells protect it inside heterocysts or root nodules.

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